Composite

Part:BBa_K571005

Designed by: Lu-Chu Ke   Group: iGEM11_TzuChiU_Formosa   (2011-10-03)


R0011+acsAB/pSB1C3

The biobrick is composite element, combination of BBa_K571000 and BBa_K571003. The promoter binding to the upstream of acsAB gene, IPTG as the inducer, activates the acsAB gene, producing Cyclic-di-GMP binding domain and UDP-forming domain. The sequence of acsAB and promoter is amplified by PCR, followed by enzyme digestion and ligation to bind the 2 sequences with the backbone-pSB1C3. The order of restriction enzyme is EcoRI, XbaI and SpeI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 666
    Illegal EcoRI site found at 2067
    Illegal PstI site found at 822
    Illegal PstI site found at 1218
    Illegal PstI site found at 2058
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 666
    Illegal EcoRI site found at 2067
    Illegal PstI site found at 822
    Illegal PstI site found at 1218
    Illegal PstI site found at 2058
    Illegal NotI site found at 3233
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 666
    Illegal EcoRI site found at 2067
    Illegal BglII site found at 1222
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 666
    Illegal EcoRI site found at 2067
    Illegal PstI site found at 822
    Illegal PstI site found at 1218
    Illegal PstI site found at 2058
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 666
    Illegal EcoRI site found at 2067
    Illegal PstI site found at 822
    Illegal PstI site found at 1218
    Illegal PstI site found at 2058
    Illegal NgoMIV site found at 2441
    Illegal NgoMIV site found at 2481
    Illegal AgeI site found at 4613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1155


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Parameters
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