Composite
Part:BBa_K571005
Designed by: Lu-Chu Ke Group: iGEM11_TzuChiU_Formosa (2011-10-03)
R0011+acsAB/pSB1C3
The biobrick is composite element, combination of BBa_K571000 and BBa_K571003. The promoter binding to the upstream of acsAB gene, IPTG as the inducer, activates the acsAB gene, producing Cyclic-di-GMP binding domain and UDP-forming domain. The sequence of acsAB and promoter is amplified by PCR, followed by enzyme digestion and ligation to bind the 2 sequences with the backbone-pSB1C3. The order of restriction enzyme is EcoRI, XbaI and SpeI.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 666
Illegal EcoRI site found at 2067
Illegal PstI site found at 822
Illegal PstI site found at 1218
Illegal PstI site found at 2058 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 666
Illegal EcoRI site found at 2067
Illegal PstI site found at 822
Illegal PstI site found at 1218
Illegal PstI site found at 2058
Illegal NotI site found at 3233 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 666
Illegal EcoRI site found at 2067
Illegal BglII site found at 1222 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 666
Illegal EcoRI site found at 2067
Illegal PstI site found at 822
Illegal PstI site found at 1218
Illegal PstI site found at 2058 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 666
Illegal EcoRI site found at 2067
Illegal PstI site found at 822
Illegal PstI site found at 1218
Illegal PstI site found at 2058
Illegal NgoMIV site found at 2441
Illegal NgoMIV site found at 2481
Illegal AgeI site found at 4613 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1155
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Categories
Parameters
None |